In vivo growth regulation during liver development in C/EBPα knockout mice. (A) Western blots of nuclear extracts isolated form fetal liver of +/+, +/−, and −/− embryos were probed with antibodies against PCNA, cyclin A (Cyc A), and β-actin. (B) BrdU incorporation in fetal liver (original magnification, 20×). A few labeled nuclei are indicated by arrows.