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. 2006 Feb;26(3):810–821. doi: 10.1128/MCB.26.3.810-821.2006

FIG. 3.

FIG. 3.

E2A gene status influences Igκ enhancer histone acetylation. (A) Chromatin modification of the Igκ enhancers in E2A−/− pre-B cells. Mononucleosomes were prepared from E2A+/+ pre-B-cell lines treated for 5 h with or without 10 μM STI-571, and acetylated H3 histones were immunoprecipitated. Relative enrichment of H3 acetylation sequences was determined by quantitative PCR and plotted as IP DNA over input DNA. Enrichment of sequences corresponding to the Igκ intronic (κEi) and 3′ (κE3′) core enhancers was determined. Enrichment of the housekeeping gene G6PD and the silent trypsinogen locus T4D served as positive and negative internal controls, respectively. Two independently derived E2A−/− clones treated with STI-571 were analyzed. (B) Mononucleosomes prepared from the pre-B cells described for panel A were immunoprecipitated and analyzed for H4 acetylated histones at various loci. Relative enrichment of acetylated H4 was plotted as IP/input. Acetyl-H4 enrichment at the G6PD and T4D loci and the Igκ enhancers (κEi and κE3′) was determined by quantitative PCR. Two independently derived E2A−/− clones treated with STI-571 were analyzed.