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. 2006 Feb;26(3):810–821. doi: 10.1128/MCB.26.3.810-821.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 7. — E2A^+/− Igκ^+/G mice have a greater proportion of GFP⁺ pre-B cells than E2A^+/+ Igκ^+/G mice. (A) Expression level of Igκ germ line transcript is not influenced by E2A gene status. Bone marrow was collected from E2A^+/+ (WT) or E2A^+/− (+/−) mice carrying one κ^o-GFP allele and analyzed by FACS. The expression levels of Igκ germ line transcript in E2A WT and heterozygous mice were determined by calculating GFP mean fluorescence intensity (MFI) among GFP⁺ (R5) pre-B cells (B220⁺ CD43^low/− IgM⁻) and immature/mature bone marrow B cells (B220⁺ CD43^low/− IgM⁺). FACS plots are representative of 12 WT and 9 heterozygous mice analyzed. APC, allophycocyanin; bio, biotin; SA, streptavidin; PE, phycoerythrin. (B) Total numbers of pre-B and immature bone marrow B cells were calculated from 12 E2A^+/+ Igκ^+/G and 9 E2A^+/− Igκ^+/G mice and plotted. (C) Percentages of GFP⁺ pre-B cells and immature bone marrow B cells from each of these mice were also calculated and plotted.