FIG. 5.

Phase resetting and temperature compensation of the period length of bioluminescence rhythms. (A) Phase resetting of the bioluminescence rhythm. The bioluminescence rhythm of the psbD- lucCP strain exposed to LD or DL was monitored under LL at 24°C. Data points and bars represent means ± standard deviations of the bioluminescence levels of 12 to 20 replicate samples. For precise phase comparisons, the maximum values of the bioluminescence on the second day were adjusted to 100, and the lower portions of the vertical axes were omitted. (B) Phase shifting of the bioluminescence rhythm. The psbD-lucCP strain on TAP agar was exposed to LD and transferred to DD at 24°C, and then a 15-minute light pulse (30 μmol m⁻² s⁻¹) was given at 3, 4.5, 7.5, or 9 h after light off (arrow heads). For phase comparisons, the maximum values on the second day were adjusted to 100, and the traces of the control without a light pulse are shown. (C) Temperature compensation of the bioluminescence rhythm. The bioluminescence rhythm of the psbD-lucCP strain on TAP agar was monitored under LL at three different temperatures. The left panel shows representative rhythms. In the right panel, data points and bars represent means ± standard deviations of the period lengths of the rhythms.