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. 2006 Feb;26(3):976–989. doi: 10.1128/MCB.26.3.976-989.2006

FIG. 5.

FIG. 5.

Characterization of part of the mouse Rad54B genomic locus and generation of mouse ES cells carrying a disrupted mRad54B allele. (A) Part of the mRad54B genomic locus and structure of the targeting construct. Exons 12 to 15 are indicated by black boxes. Shown are the locations of selected restriction sites, EcoRI (E), BamHI (B), BglII (Bg), HindIII (H), and XbaI (X). The positions of two different probes, named A and B, are indicated. (B) DNA blot analysis of G418-resistant ES clones with probe A and EcoRI digested DNA. The wild-type (wt) allele yields a 3.0-kb band, while the disrupted allele results in a 3.6-kb band. Lane 1, wild-type ES cell; lane 2, clone with a randomly integrated targeting construct; lane 3, clone with a homologously integrated targeting construct. (C) RNA blot analysis of mRad54B transcripts in mice carrying the disrupted allele. Total RNA (15 μg) isolated from testes of wild-type, mRad54B+/−, and mRad54B−/− males was probed with 5′ and 3′ mRad54B cDNA probes. A GAPDH (glyceraldehyde-3-phosphate dehydrogenase) cDNA probe served as a loading control.