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Wild-type and mutant Rad1 proteins purified from yeast: wild-type protein (lane 1) or mutant Rad1 proteins K750 N752 and K749, respectively (lanes 2 and 3). A 0.5-μg aliquot of each protein was electrophoresed on a denaturing 7.5% polyacrylamide gel and stained with Coomassie brilliant blue.
