FIG. 1.

ADFP gene targeting. (A) ADFP partial genomic structure, gene targeting construct, and expected homologous recombinant allele. Restriction enzyme sites: A, AccI; B, BamHI; E, EcoRI; X, XbaI; and Xh, XhoI. (B) Southern blot genotyping of the wild type (+/+) and heterozygous (+/−), and homozygous (−/−) knockouts. (C) Northern blot analysis of RNA from four different tissues (L, liver; M, muscle; (B) brain; and W, white adipose tissue) in wild-type and homozygous knockouts. (D) Western blot of total protein extracted from wild-type and homozygous knockout livers.