FIG. 7.
(A) In vivo hepatic fatty acid synthesis using the [3H]H2O labeling method (39, 40). Male Adfp+/+ and Adfp−/− mice (four mice per group) were synchronized to 3-h (8 to 11 a.m.) feeding each day for 2 weeks before experiments. Livers were removed 1 h after [3H]H2O injection. Total lipids were extracted, saponified, and separated by TLC; fatty acid fractions were scraped into scintillation vials to determine radioactivity. (B) Liver FAS activity of liver extracts from Adfp+/+ and Adfp−/− mice (four mice per group). (C) Representative Western blot of liver FAS and ACCI from Adfp+/+ and Adfp−/− mice. (D) Liver microsomal DGAT activity assay (Materials and Methods) of microsome preparations from Adfp+/+ and Adfp−/− mice (four mice per group). The reaction products from the DGAT assay were separated by TLC, the TG fraction was scrapped, and radioactivity was determined by scintillation counting.