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. 2006 Feb;26(3):1063–1076. doi: 10.1128/MCB.26.3.1063-1076.2006

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FIG. 8. — TLC analysis of cytosolic and microsomal lipid distribution. A total of 0.4 g of liver from Adfp^+/+ and Adfp^−/− mice (four mice per group) was homogenized and separated into nuclear, microsomal, and cytosolic compartments. Lipids were extracted from each compartment and analyzed by TLC. Two lipid standards were loaded in the center lanes as a control. (B) Western blot analysis of subcellular fractions. A total of 20 μg of proteins from each subcellular fraction (prepared as described for panel A) was analyzed by PAGE, transferred to a nylon membrane, and immunoblotted with anti-MTP (microsomal marker), anti-GAPDH (cytosolic marker), and antihistone (nuclear marker). T, total liver protein extract; C, cytosolic fraction; N, nuclear fraction; M, microsomal fraction. +/+, Adfp^+/+ mice; −/−, Adfp^−/− mice. (C) Intracellular lipid partitioning in Adfp^−/− and Adfp^+/+ mice. Lipid (TG, NEFA, and PL) abundance was analyzed by TLC, quantified by densitometry, and expressed according to each lipid's subcellular compartment.