FIG. 2.

Analysis of different 3" downstream regions on snoRNA processing. The rna15-2 and pap1-5 mutant strains and the isogenic strain W303 were transformed with the constructs indicated above each panel. After a 1-h shift to the nonpermissive temperature (0 h), cultures were induced with galactose for the indicated times. Northern blot analysis of 5 μg of total RNA was performed with a ³²P-labeled tag-specific oligonucleotide (αtag). A schematic representation of the A⁺, B, and U24* molecules is given on the far right. A⁺ bands correspond to polyadenylatedproducts, while B and U24* molecules have mature 3" ends. The lower panels show control hybridizations with a snR13 snoRNA-specific probe (αsnR13). The migration of the size marker (MspI-digested pBR322) is indicated on the far left; sizes are in nucleotides.