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Kin28 mutants are not sensitive to UV light-induced DNA damage. Plates containing the indicated Kin28 and Tfb1 mutants were exposed to increasing doses of short-wave UV (200 μW per s per cm2), and surviving colonies were counted. The resulting viability at each time point is expressed as a percentage of the untreated control for each strain. UV-sensitive Tfb1 carboxy-terminal truncation mutants (products of tfb1-6 and tfb1-101 [49]) were used as controls. WT, wild type.
