FIG. 6.

Complex I is a trimer of Kin28, Ccl1, and Tfb3. Immunopurified complex I (ProtA:α-HA) was subjected to SDS-PAGE followed by silver staining and immunoblotting analysis. As a negative control, the Ni-NTA flowthrough fraction for complex I was precipitated without the anti-HA antibody (ProtA). The positions of protein A and the immunoglobulin heavy and light chains (IgH and IgL, respectively, are indicated. Immunoblotting with antibodies against Ccl1, Tfb3, or Kin28 shows that these proteins align with silver-stained species of the appropriate size. Note that the immunoglobulin proteins are also detected in the anti-HA immunoblot.