FIG. 7.

Functional analysis of complex I trimer and TFIIH from the T162 mutants. Trimer and TFIIH complexes were biochemically purified from strains containing wild-type (WT) Kin28 or the T162A or T162D mutants as described for Fig. 5 and in Materials and Methods. Following the final purification via the HA epitope tag, the resulting complexes were subjected to SDS-PAGE and immunoblot analysis (Western; top). The immunoprecipitates were also used for an in vitro CTD phosphorylation assay (bottom). GST-CTD*P, phosphorylated GST-CTD.