TABLE 1.
Yeast strains used in this study
| Strain | Genotypea |
|---|---|
| YSB207 | MATatfb1Δ::LEU2 ura3-52 leu2-3,112 his3Δ200 [pRS316-TFB1] |
| YSB251 | MATatfb1Δ::LEU2 ura3-52 leu2-3,112 his3Δ200 [pRS313-tfb1-6] |
| YSB260 | MATatfb1Δ::LEU2 ura3-52 leu2-3,112 his3Δ200 [pRS313-tfb1-101] |
| YSB347 | MATα kin28Δ::LEU2 ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 ade3-22 can1-100 [YCplac22-KIN28] |
| YSB591 | MATα kin28Δ::LEU2 ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 ade3-22 can1-100 [pRS426-KIN28] |
| YSB722 | MATakin28Δ::LEU2 tfb1Δ::LEU2 ura3 leu2-3,112 trp1-1 his3 ade2-1 [pRS426-KIN28; pRS313-TFB1-6his] |
| YSB759 | MATakin28Δ::LEU2 tfb1Δ::LEU2 ura3 leu2-3,112 trp1-1 his3 ade2-1 [pRS314-haKIN28; pRS313-TFB1-6his] |
| YSB760 | MATakin28Δ::LEU2 tfb1Δ::LEU2 ura3 leu2-3,112 trp1-1 his3 ade2-1 [pRS314-hakin28(T162A); pRS313-TFB1-6his] |
| YSB761 | MATakin28Δ::LEU2 tfb1Δ::LEU2 ura3 leu2-3,112 trp1-1 his3 ade2-1 [pRS314-hakin28(T162D); pRS313-TFB1-6his] |
| YF82 | MATa/MATα KIN28/kin28Δ::LEU2 ura3-1/ura3-1 leu2-3,112/leu2-3,112 trp1-1 his3-11,15/his3-11,15 ade2-1/ade2-1 ade3-22/ade3-22 can1-100/can1-100 |
a
Brackets indicate episomal plasmids.