TABLE 2.
Growth phenotypes of Kin28 mutants
| Mutant class | Amino acid change(s) | Growth phenotypea |
|---|---|---|
| NAb (wild type) | None | +++ |
| Catalytic site | K36A | + |
| E54Q | − | |
| D147N | − | |
| Potential inhibitory phosphorylation site | T17V, -E, or -Q | +++ |
| T17D | + | |
| Y18F | +++ | |
| T17V, Y18F | +++ | |
| Potential activating phosphorylation site | T162A, -D, -V, -E, or -Q | +++ |
| S163A | +++ | |
| T162A, S163A | +++ | |
| Double | T162A, T17D | − |
| T162A, K36A | − |
a
+++, wild-type growth; +, slow growth; −, inviable. The same results were obtained at 16, 23, 30, and 37°C, as well as when galactose was substituted for glucose in the media.
b
NA, not applicable.