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O/E-1, -2, and -3 mRNAs are expressed in mouse adipose tissue. Total RNA was extracted from the visceral adipose tissues and livers from five animals and from the 70Z/3 B-lymphoid cell line. After cDNA synthesis with DNase-treated RNA samples, transcript levels of O/E-1, -2, and -3 were analyzed by quantitative real-time PCR.
