FIG. 4.

mRNA levels of adipocyte differentiation markers during normal and O/E-1-stimulated 3T3-L1 differentiation. Cells infected with retroviruses containing empty vector or O/E-1 cDNA were induced to differentiate by the addition of dexamethasone and darglitazone 2 days after confluence. Total RNA was extracted from cultures at preconfluence (lanes P), confluence (lanes C), and days 1, 2, and 4 postinduction (lanes 1, 2, and 4, respectively). cDNAs were synthesized from DNase-treated samples and used in a quantitative real-time PCR. The results of one representative experiment out of four are shown.