FIG. 7.

A dominant negative (DN) O/E-1-Engrailed fusion protein represses O/E-1-dependent target promoters. (A) Schematic drawing of the O/E-1-Engrailed construct. Amino acids 1 to 430 of mouse O/E-1 were N-terminally fused to amino acids 1 to 298 of the Drosophila Engrailed protein. DBD, DNA binding domain. (B) The O/E-1-Engrailed hybrid cDNA was cloned into the pcDNA3 expression vector and used in cotransfection experiments. 70Z/3 pre-B cells were transfected with empty pcDNA3 (vector) or pcDNA3 containing O/E-1-Engrailed (O/E-Engrailed) in conjunction with reporter plasmids containing the luciferase gene driven by the mb-1, B29, or CMV promoter. The resulting luciferase activities from three transfections are indicated.