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. 2002 Nov;22(22):7744–7757. doi: 10.1128/MCB.22.22.7744-7757.2002

FIG. 2.

FIG. 2.

Cellular localization of Ypt11p. (Top) ypt11Δ cells (strain yTO001) with pK003 (single copy), a low-copy-number plasmid carrying HA-YPT11, which produces Ypt11p tagged with HA at its N terminus under the control of the YPT11 promoter, or with pK004 (multicopy), a high-copy-number plasmid carrying HA-YPT11, were grown in SC medium lacking uracil, fixed, and stained by the indirect-immunofluorescence method using anti-HA mouse antibodies (16B12) and FITC-conjugated anti-mouse IgG. (Middle) Wild-type cells (strain YPH499) with pK004 and a GFP-tagged version of MYO2, replacing the wild-type MYO2 allele, were grown in SC medium lacking uracil, fixed, stained by the indirect-immunofluorescence method using anti-HA rat antibodies (HA-Ypt11) and anti-GFP mouse antibodies (Myo2-GFP), and visualized with Alexa 546-conjugated anti-rat IgG and FITC-conjugated anti-mouse IgG, respectively. Phase-contrast images are shown on the left. (Bottom) Wild-type cells (strain YPH499) with pK004 were grown in SC medium lacking uracil, fixed, and stained by rhodamine-phalloidin (actin) and the indirect immunofluorescence method using anti-HA mouse antibodies (16B12) and FITC-conjugated anti-mouse IgG (HA-Ypt11). Phase-contrast images are shown on the left. Bars, 5 μm.