FIG. 7.

Synthetic growth defect of myo2-573 with ypt11Δ. myo2-573/+ ypt11Δ/+ heterozygous diploid cells were sporulated, and three sets of tetrads (1 to 3), dissected, are shown in the upper panel. The genotypes of the spore clones are indicated in the lower panel: wild-type spores (open circles), ypt11Δ spores (gray circles), myo2-573 spores (black circles), and myo2-573 ypt11Δ spores (squares). The myo2-573 spore clones did not form visible colonies after incubation for 3 days at 25°C in combination with ypt11Δ, whereas ypt11Δ MYO2 spore clones and myo2-573 YPT11 spore clones did so. After prolonged incubation, several myo2-573 ypt11Δ spore clones formed microcolonies, which were difficult to repropagate.