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GILZ overexpression inhibits c-Fos but not c-Jun trascription. Western blot analysis of c-Fos (A) and c-Jun (B) expression was performed. Nuclear cell lysates (10 μg) from PV6 and GIRL-19, stimulated for 1 h with immobilized anti-CD3 MAb (1 μg/ml), were probed with anti-c-Fos or anti-c-Jun antibodies (1 μg/ml). C, untreated cells. (C) The expression level of endogenous and exogenous GILZ was evaluated by Western blotting with anti-GILZ antiserum.
