FIG. 9.

DEX inhibits Raf-1, MEK, and ERK-1/2 phosphorylation. Mouse thymocytes left untreated or pretreated for 6 h with DEX (100 nM) were stimulated for 1 h with plastic-bound anti-CD3 MAb. Western blotting was performed with an anti-pERK-1/2 (A), anti-pMEK-1/2 (B), anti-pRaf-1 (C), or anti-GILZ (D) antibody. Western blotting was also performed with an anti-ERK-1/2, anti-MEK-1/2, or anti-Raf-1 antibody to verify that no modulation of protein expression occurred or with β-tubulin to verify that equivalent amounts of proteins were loaded in all lanes.