FIG. 4.

Transcriptional activation of the bcl-2 promoter in HepG2 cells. Cells were transfected with 1 μg of −670 Bcl-2 CAT construct in the presence of 4 μg of the pSV-RB expression vector (cross-hatched and black bars) or the empty expression vector (white and stippled bars) and 4 μg of the pPADH-AP2 expression vector (stippled and black bars) or the corresponding empty expression vector (white and cross-hatched bars). Values were obtained from two independent experiments, each analyzed in duplicate. The results are averages expressed as fold activation of CAT activity relative to the basal promoter activity, which is assigned a value of 1. Error bars indicate standard deviations.