FIG. 7.

In vivo recruitment of RB and AP-2 proteins and histone acetylation on the bcl-2 promoter in MCF7 and C33 cells. Graphic representation of results obtained by real-time PCR as described in the legend to Fig. 6. The values are expressed as a fraction of the total number of copies (input) detected as antibody-bound material (percentage of input multiplied by 100). Averaged values were obtained from three independent experiments, each analyzed in triplicate. Error bars indicate standard deviations of the means. Equal amounts of chromatin extracted from MCF7 (A) and C33 (B) cells were subjected to immunoprecipitation using anti-RB (black bar), anti-AP-2 (grey bar), or anti-AcH4 (cross-hatched bar) antibodies. bcl-2 sequence (Bcl-2 gene) was detected by real-time quantitative PCR. A sample immunoprecipitated with an anti-mouse IgG antibody (IgG) (white bars) was used as a negative control and revealed no significant association with the bcl-2 promoter. The GAPDH sequence (GAPDH gene) was also used as a negative control.