TABLE 1.

Suppression of RB-mediated activation of the bcl-2 promoter by dominant-negative AP-2

Activation by:	Amt of dominant-negative AP-2 (μg)	Promoter activity
		−670 Bcl-2a	−134 Bcl-2b
RB	0	100.0 ± 0.5
	1	20.8 ± 2.8
	2	6.4 ± 3.6
Basal level	0		100.0 ± 0.5
	1		99.5 ± 0.2
	2		89.5 ± 6.3

^aOne-half microgram of −670 Bcl-2 CAT was transfected into MDCK cells either with 2.5 μg of pUC DNA or with pSV-RB expression vector, as indicated, plus increasing amounts of dominant-negative AP-2 in which the N-terminal region has been deleted. The activity of the −670 Bcl-2 CAT reporter in the presence of the control vector without the AP-2 sequence was set at 100%.

^bThe negative control −134 Bcl-2 CAT vector, which does not contain the AP-2 binding site, was used. Its basal level was determined in a cotransfection assay with increasing amounts of dominent-negative AP-2.