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Activity of mce1R′-′lacZ translational fusions. (A) Schematic representation of the genomic regions cloned into pIGn to create the mce1R′-′lacZ translational fusions, pIGr3 and pIGr6. (B) β-Galactosidase activity in cell lysates of M. smegmatis transformed with the promoter constructs. The vector pIGn contains no promoter. Values represent the mean activities of three colonies (nmol ONP mg−1 min−1), and error bars show 1 standard deviation.
