FIG. 4.

Sequence analysis of Mce1R. (A) Unrooted phylogenetic tree of GntR family regulators, showing division of the four subfamilies, FadR, YtrA, MocR, and HutC (underlined). Organism abbreviations are as follows: as, Acinetobacter sp. strain ADP1; bh, Bacillus halodurans; bs, Bacillus subtilis; ec, E. coli; mt, M. tuberculosis; pp, Pseudomonas putida; rl, Rhizobium leguminosarum; rm, Rhizobium meliloti; rs, Rhodobacter sphaeroides; sa, Staphylococcus aureus; sl, Streptomyces lividans; and st, Salmonella enterica serovar Typhimurium. (B) Secondary-structure-based alignment of Mce1R with GntR and FadR. Predicted α-helical regions are highlighted in gray and β-strands in black. The structural elements of FadR determined from its crystal structure are shown above the alignment. The helix-turn-helix DNA-binding motif is shaded.