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Effect of overexpression of Mce1R on transcription of mce1 genes. RT-qPCR analysis of mce1 gene expression in WT (white bars) and attB::glnA1p′-mce1R (pCER17; gray bars) in 7H9 medium. Results are presented as the ratio of the amount of target gene in the recombinant strain to the amount in the WT strain for each gene. Values shown are means of triplicate PCRs, and error bars represent 1 standard deviation.
