TABLE 1.
Oligonucleotide sequences
| Procedure | Gene | Forward primer | Reverse primer | TaqMan probe |
|---|---|---|---|---|
| RT-PCR | Rv0165c | CCACGTGAACGCACCTCTATC | AGCAGGAACCAGGCCAACTTG | |
| Rv0166 | TGCTGGAGGCAGAGAAGGTC | GGGTTGTTCCAGTAGCAGCTC | ||
| Rv0167 | ATGGTCTCGATACCGCTGACG | CACCTCCATCGCGTCGATTTC | ||
| Rv0168 | GCTTTGACCAGCATCGG | CACCGAGACCAACGAGAAACG | ||
| Rv0169 | CACCATCTCGGAGGTCACACG | CGCTCAGGGTCAGGTTCAGC | ||
| Rv0170 | AGTTCAGCAATGTCAGCGGG | GTCCAGCACGATGTTCAGGTT | ||
| Rv0171 | TCGCTGAATGTGTTGTCGGAG | CGGGTTGTCGTTGATAAGGTTTTG | ||
| Rv0172 | GTCGCGGAACATTCAGTTGG | GAATGCCTCGATGACTTCGC | ||
| Rv0173 | CTCTTGCTGAGTTCGTGCGG | GGTGGCATTTTTCGGTAGCG | ||
| Rv0174 | GCGCTGGACAATTCCAATCG | CGGAGTTCTCGATGATGTCG | ||
| Rv0175 | GGCTAGCATGCAGAAGATCATCG | CCTCATCCAGTGCCATCCG | ||
| Rv0176 | GCGCACTTGGTGGACACC | GATCGGTTTGCCATTCATTGACG | ||
| Rv0177 | CTGGCTCTGACCGCATCC | TTCCTGTGGTCGGTTCCACC | ||
| Rv0178 | GTTCGTGGGCTCGGCAG | GATCGGCCAGGGTGTCC | ||
| RT-qPCR | sigA | CCGATGACGACGAGGAGATC | GGCCTCCGACTCGTCTTCA | AAGGACAAGGCCTCCGGTGATTTCGa |
| fadD5 | CTTAACCGCACCGAGTTCGT | TGAGCCGGAAATTCAGTGGTA | TCGGTGCTGGCCGCCAACAb | |
| mce1A | TGCACGATCCGCAACTTCT | TCCTCAGCGAGTAGCCGTTAC | CGATGCCGATCCGCTCGCTAAAGb | |
| mce1F | CGTCGGTGACTTCAAAACCA | TTGACCTGGCTGTCCAAAATC | TTGGCGACGTCAACGACATCATCGb |
a
5′, 6-carboxyfluorescein; 3′, 6-carboxytetramethylrhodamine (TAMRA).
b
5′, VIC; 3′, TAMRA.