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. 2006 Jan;188(2):361–369. doi: 10.1128/JB.188.2.361-369.2006

FIG. 1.

FIG. 1.

The arcABC operon of S. suis consists of the arcA, arcB, and arcC genes. (A) Schematic representation of the positions of the primer sequences used for RT (rev1 to rev3) and the primers used for PCR (P1 to P14) as described in Materials and Methods. (B) Ethidium bromide-stained gel from the RT-PCR analysis of mRNA of S. suis grown in THB at 37°C. Lane 1, molecular weight marker; lane 2, flpS intragenic PCR fragment (603 bp, primer pair 1); lane 3, flpS upstream region 3′-end flpS PCR fragment (primer pair 2); lane 4, arcA intragenic PCR fragment (229 bp, primer pair 3); lanes 5 and 6, flpS-arcA gene intergenic PCR fragment (primer pair 4 each); lane 7, arcA-arcB intergenic PCR fragment (2,132 bp, primer pair 5); lane 8, arcB-arcC intergenic PCR fragment (1,621 bp, primer pair 6); lane 9, arcD intragenic PCR fragment (527 bp, primer pair 7); lane 10, arcC-arcD intergenic PCR fragment (primer pair 8); lanes 11 and 12, control reactions with mRNA without RT and double-distilled H2O, respectively.