FIG. 4.

Real-time RT-PCR to study the effects of pLSV101 insertion on the transcription of genes located downstream. cDNAs obtained from wild-type strain EGD (Wt Egd) and mutant EGD-lmo1971::pLSV101 (lmo 1971 :: pLSV 101) were used as a template for real-time RT-PCR with oligonucleotides specific for lmo1968. The quantity of lmo1968-specific cDNA was normalized to the quantity of rpoB cDNA, indicating that the concentration of lmo1968-specific cDNA and thus mRNA is not reduced in the mutant strain.