TABLE 1.
Bacterial strains used in this study
| Strain | Characterization | Source or reference |
|---|---|---|
| E. coli | ||
| XL2-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tetr) Amy Camr] | Stratagene |
| DH5α | deoR endA1 gyrA96 hsdR17(rK− mK+) recA1 relA1 supE44 λthi-1 Δ(lacZYA-argF)U169 | 18 |
| L. monocytogenes | ||
| EGD | Sv1/2a, wild-type | S. H. E. Kaufmann |
| EGD-e | Derivative of EGD | G. B. Mackaness |
| Pkp1 | Sv1/2a ΔplcA-hly-mpl-actA-plcB, Kanr | 10 |
| EGDΔglpD (lmo1293) | In-frame deletion of glpD (lmo1293) | This study |
| EGDΔ1538 | In-frame deletion of lmo1538 | This study |
| EGDΔ1538ΔglpD (lmo1293) | Double mutant of lmo1538 and glpD (lmo1293) | This study |
| EGD-eΔ1968-1974 | In-frame deletion of the gene cluster lmo1968-1974 specific for L. monocytogenes | This study |