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CMM of heat shock (hs)-induced FLW-1/Fab71 is transcribed two- to threefold more compared with non-heat-shocked controls. A digoxigenin-labeled 3.6-kb Fab7 RNA probe (distal-proximal [d-p] orientation) was hybridized to a slot blot of 5 μg of polyadenylated RNA of various lines. The endogenous Fab7 locus was transcribed weakly in the wild type (wt). Additional controls included 10 ng of in vitro-transcribed Fab7 RNA.
