FIG. 5.

Host lysis by chimeric Mu prophage bearing ECs-based sites. The central region of the Mu genome carrying the SGS was replaced by either the wild-type ECs site or a derivative with substituted sequence in the left (ECsΔL) or right (ECsΔR) arms. Host E. coli cells carrying one of these prophages were grown at 30°C, and Mu replication was induced by a temperature shift to 42°C. Wild-type Mu prophage (SGS) or one lacking the entire SGS (ΔSGS) was included as a control. Host lysis was followed by Klett readings of the induced cultures.