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Detection of native and recombinant DraSO activities on a 7.5% nondenaturing gel. Samples were stained for sulfite oxidase activity after electrophoresis under alkaline conditions and equilibration of the gel in the assay buffer. Lane 1, recombinant His6-tagged DraSO after purification on nickel affinity gel; lane 2, crude extract of D. radiodurans; lane 3, Coomassie brilliant blue staining of purified recombinant DraSO.
