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. 2006 Jan;188(2):794–800. doi: 10.1128/JB.188.2.794-800.2006

FIG. 2.

FIG. 2.

Biosynthesis of (p)ppGpp in V. cholerae relaxed mutants. Left panel, the stringent response was induced as described in the text; radiolabeled guanine nucleotides were analyzed by TLC and detected by autoradiography. Lane 1, C7258; lane 2, AJB19; lane 3, CF1648; lane 4, CF1652 (relA251). Right panel, 10 μl of three independent single-colony cultures of C7258 and AJB19 grown in LB medium were spotted in M9 minimal medium, M9 medium supplemented with AT, and M9 medium supplemented with SMGL and incubated 24 h at 37°C.