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. 2002 Nov;22(22):7907–7918. doi: 10.1128/MCB.22.22.7907-7918.2002

FIG.1.

FIG.1.

Replication of Py ori core DNA by acetyltransferases. (A) Structures of test plasmids used to measure replication stimulation. (B) Structures of acetyltransferases fused to GalDBs. Acetyltransferase (HAT) domains are indicated by dark bars. Two HAT substitution mutants of human GCN5 (FTE and DEY), one HAT deletion mutant of PCAF (Δ574-608), and one deletion mutant of PCAF in the p300/CBP interacting domain (Δ65-112) also are indicated. aa, amino acid. (C) Examples of replication assays performed with the test plasmids pBSPyGal and pBSPyΔE in FOP cells. Test plasmids and cotransfected expression vectors are indicated above lanes. The arrow and the asterisk indicate the positions of replicated test DNA and the nonreplicated test DNA (input), respectively. (D) Averages from three independent replication assays (means ± standard deviations). (E) Examples of replication assays performed with the test plasmids pBSPyGal and pBSPyΔE in NIH 3T3 cells. Test plasmids and cotransfected expression vectors are indicated above lanes. The arrow and the asterisk indicate the positions of replicated test DNA (top band) and the nonreplicated test DNA as a reference (lower band), respectively. (F) Averages from three independent transfection assays (means ± standard deviations).