FIG. 4.

In vitro acetylation of PyLT by PCAF. (A) PyLT incubated with (+) or without (−) PCAF with [³H]acetyl-coenzyme A as described in Materials and Methods. The right-hand panel shows BSA incubated with PCAF as a control. Reaction products were separated by SDS-PAGE and stained with Coomassie blue, and [³H]acetate reaction products were visualized by fluorography. (B) PyLT incubated with acetyl-coenzyme A and PCAF or p300 detected by Western blotting by using rabbit anti-acetyl-lysine polyclonal antibody (α acetyl lysine). Upper panel, Western blot with anti-α-acetyl-lysine antibody; lower panel, Coomassie-stained gel.