FIG. 2.
Map of the DNA constructs cloned into IS256L of Tn4001mod of pKV74 or Tn4001cat of pKV104 to create pKV258, pKV264, pKV265, and pKV299. Except for the IS elements, all open reading frames are shown with the 5′ end on the left. The region encoding the 3′ end of MPN140 is not indicated. B, BamHI; Bs, BsaBI; E, EcoRI; S, StuI; Sm/P, SmaI/PmeI junction; M/E/Sp, former SphI site of the P1 operon replaced by MfeI linker and present as an MfeI/EcoRI junction; E or Sm, EcoRI in pKV74 or SmaI in pKV104. The barred symbol indicates a silent change to destroy the site. Gentamicin resistance (Gmr) is found in pKV74, and chloramphenicol resistance (Cmr) is found in pKV104. The arrow from Tn4001mod/cat shows the location and direction of the POUT promoter. Note that the orientation of the inserts is inverted when cloned into Tn4001mod/cat.