TABLE 1.
Strains examined in this study
| Strain | Relevant genotypea | Relevant phenotypeb
|
Source or reference | |
|---|---|---|---|---|
| P1 (MPN141) | B/C (MPN142) | |||
| M129 | Wild type | ++ | ++ | 17 |
| III-4 | MPN142 frameshift | ++ | − | 17, 30 |
| IV-22 | MPN141 frameshift | − | − | 17, 27 |
| III-4 + pKV258 | III-4 + MPN141 and MPN142* | ++ | ++ | 30 |
| III-4 + pKV264 | III-4 + MPN142 | ++ | ++ | 30 |
| III-4 + pKV265 | III-4 + del MPN141† and MPN142 | ++ | + | This study |
| IV-22 + pKV258 | IV-22 + MPN141 and MPN142 | ++ | ++ | This study |
| IV-22 + pKV264 | IV-22 + MPN142 | − | +/− | This study |
| IV-22 + pKV265 | IV-22 + delMPN141† and MPN142 | − | − | This study |
| IV-22 + pKV299 | IV-22 + MPN141 | ++ | +/− | This study |
| IV-22 + pKV299 and pKV264 | IV-22 + MPN141 and MPN142‡ | ++ | ++ | This study |
a
*, All restored genes are recombinant and delivered by transposons borne by the indicated plasmids; †, the 5′ end of MPN141 is removed, rendering it untranslatable; ‡, MPN141 and MPN142 were delivered by different transposons.
b
++, Protein present at or near wild-type levels; +, protein present at reduced levels; +/−, protein present at trace levels; −, protein undetected.