FIG. 1.

Restriction maps and RT-PCR analyses. (A) Restriction maps of the pdeA, pdeB, and pdeC genes of M. xanthus. Lines with arrows indicate the orientation. (B) RT-PCR analysis for polar effects. Total RNA was prepared from each of the strains, mutant and wild-type (WT) strains. The RNA (0.2 μg) was treated with (+) or without (−) reverse transcriptase and subjected to PCR with appropriate primers. (C) Cotranscription of pdeA and orfB or pdeB and orfC in the wild-type strain. Total RNA prepared from vegetative cells of the wild type was either treated (+) or not treated (−) with reverse transcriptase. PCRs were performed using pdeA-orfB primers and pdeB-orfC primers. The positions of molecular size markers (base pairs) are indicated to the left of the gels.