TABLE 4.

Expression of ICE-lacZ and Fur box-lacZ reporter fusions in S. cerevisiae strains synthesizing Gal4′-′Irr or Gal4′-′Fur hybrid prey proteins^a

Yeast strain	Target	β-Galactosidase activity (Miller units)
		Gal4′-′Irr	Gal4′-′Fur
8730	ICEhmu	36.1 ± 9.7	0.0
8732	ICEhmumut	0.0	ND
8733	ICE6680	10.1 ± 1.3	0.0
8734	ICE7895	93.5 ± 8.4	0.0
8765	Fur box	ND	5.3 ± 0.3

^aCells of yeast reporter strains were transfected with prey plasmid pRJ8737 (Gal4′-′Irr) or pRJ8735 (Gal4′-′Fur). Cultures grew in synthetic dropout medium (−URA, −LEU) for 3 to 5 h until they reached mid-exponential phase (optical density at 600 nm of 0.5 to 0.8), and one-hybrid interactions were examined as β-galactosidase activity. Activity was measured from two cultures per strain, each assayed in duplicate. Control yeast strains 8730, 8733, 8734 and 8765 harboring the vector pGAD424 (encoding Gal4′ only) showed no β-galactosidase activity. Data are shown for one out of four independent experiments, all yielding similar results. ND, not done.