FIG. 1.
Scc, a coiled-coil protein from Leptospira spp. (A) Genetic organization of the rplT locus in E. coli and L. interrogans. Constructs used for the generation of L. biflexa mutants are also indicated. Kmr, kanamycin resistance cassette. (B) Sequence alignment of Scc proteins of L. interrogans (Li nt) and L. biflexa (Lbif). The arrow maps the frameshift in L. biflexa. Residues conserved in the two proteins are shaded. The a and d positions of the heptad repeat of the putative coiled-coil domain are shown below the alignment.