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. 2006 Jan;188(2):469–476. doi: 10.1128/JB.188.2.469-476.2006

FIG. 6.

FIG. 6.

Nucleic acid binding assays of Scc. (Left) Electrophoretic mobility shift assays of L. biflexa Scc. Agarose gel electrophoresis staining with ethidium bromide for visualizing DNA (A) and RNA (B). Lanes 1 and 7, 1 μg plasmid DNA (A) or 1 μg total RNA from L. biflexa (B). Lanes 2 to 6, 1 μg of plasmid DNA (A) or total RNA (B) with 2, 5, 10, 20, and 40 μg of Scc-6His protein. All reactions were carried out in a total volume of 40 μl. (Right) Real-time surface plasmon resonance (SPR) profiles showing the interaction between immobilized L. biflexa Scc-GFP-6His and 250-μg/ml plasmid DNA (A) or 150-μg/ml total RNA (B) in solution. No binding was observed on GFP or truncated Scc (95-212)-GFP-6His.