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TLC analysis of acyl-HSL produced by parent strain P. aeruginosa PAO1 and its rsaL-negative mutant derivative visualized by overlaying with acyl-HSL sensor strain E. coli(pSB1075) (30). Lane 1, synthetic 3OC12-HSL and 3OC10-HSL (0.8 μg); lane 2, PAO1; lane 3, PAO1 rsaL; lane 4, PAO1 rsaL carrying plasmid pPSRsaLPAO. Acyl-HSLs were extracted from spent supernatants, and a volume corresponding to 8 × 108 CFU was loaded for the TLC assay.
