FIG. 8.

Presence of d-alanine in S. aureus LPXTGase. ¹⁴C-labeled S. aureus LPXTGase was prepared, and [¹⁴C]alanine was purified from 6 N HCl hydrolysate of the ¹⁴C-labeled enzyme. [¹⁴C]alanine was subjected to a d-amino acid oxidase reaction, and reaction products were separated on silica gel TLC plate using chloroform-methanol-water (1:2:1) as a running solvent. Radioactive products were detected by radioautography. Lane 1: l-alanine. Lane 2: l-alanine treated with d-amino acid oxidase. Lane 3: alanine from LPXTGase. Lane 4: alanine from LPXTGase treated with d-amino acid oxidase. Lane 5: pyruvic acid.