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Resistance of LPXTGase to proteolysis. About 22 μg each of intact or deglycosylated S. aureus LPXTGase was incubated with either trypsin or lysostaphin as described in Materials and Methods. Untreated and protease-treated intact or deglycosylated LPXTGase samples were labeled with FITC, and the labeled products were chromatographed on silica gel thin-layer plates as described in Materials and Methods. Trypsin and lysostaphin remained at the origin under the same TLC conditions.
