TABLE 1.
Effects of salts on the activities of LPXTGases from S. aureus and S. pyogenes
| Salt (concn [mM]) | Peptide cleaved, cpm (% of control)
|
|
|---|---|---|
| S. aureus | S. pyogenes | |
| Sodium chloride (100) | 6,190 (14.1) | 6,720 (16.2) |
| Potassium chloride (100) | 5,820 (13.2) | 8,608 (20.7) |
| Ammonim acetate (100) | 27,900 (63.6) | 22,380 (53.9) |
| Hydroxylamine, HCI (100) | 12,160 (27.4) | 11,200 (27.0) |
| Calcium chloride (20) | 8,220 (18.6) | 9,706 (23.4) |
| Magmesium chloride (20) | 6,160 (14.0) | 8,615 (20.7) |
| Potassium phosphate (20) | 23,920 (54.2) | NDa |
| Sodium phosphate (20) | ND | 20,646 (49.7) |
| Sodium sulfate (20) | 22,920 (52.0) | 23,454 (56.5) |
| Ammonum sulfate (20) | 49,930 (113.4) | 42,040 (101.2) |
| Sodium bicarbonate (20) | 54,080 (122.8) | 52,610 (126.7) |
| Ammonum bicarbonate (20) | 70,360 (159.8) | 58,890 (141.8) |
| EDTA (5) | 12,360 (28.2) | 2,147 (5.2) |
| Control; no salt | 44,040 (100) | 41,530 (100) |
a
ND, not determined.