FIG. 6.
(A) Distribution of observed rupture forces for pili force spectra over mica surfaces. A mean rupture force of 95 pN was obtained. (B) Distribution of corresponding piezo retraction distances needed for rupture. Corresponding pili lengths depend on the (unknown) positions of the bacteria on the AFM cantilever. (C) Distribution of piezo retraction distances for bond rupture for poly-l-lysine-coated AFM tip. No rupture lengths in excess of 600 nm were observed. The inset shows a typical force spectrum for poly-l-lysine. (D) Distribution of piezo retraction distances for bond rupture for strain PAK-Δpil without pili. No rupture lengths in excess of 100 nm were observed. The inset shows a typical force spectrum for strain PAK-Δpil.
