TABLE 3.
Growth of E. coli and S. enterica strains on fatty acids of various chain lengths
| Strain | Growth on indicated carbon sourceb
|
||||
|---|---|---|---|---|---|
| C18:1 | C10 | C8 | C6 | C2 | |
| E. coli UB1005 | 5+ | −a | − | − | 5+ |
| S. enterica LT2 | 5+ | 2+ | − | − | 5+ |
| E. coli UB1005 fadR | 5+ | 5+ | − | − | 5+ |
| S. enterica LT2 fadR | 5+ | 5+ | 2+ | 1+ | 5+ |
Isolated colonies (characteristically fadR mutants) appeared following extended incubation (>4 days) at 37°C.
Growth of pinpoint colonies was visible after extended incubation (>5 days) at 37°C. The cultures were grown at 37°C on plates of M9 medium supplemented with oleate (C18:1), decanoate (C10), octanoate (C8), hexanoate (C6), or acetate (C2). After 7 days of incubation, growth was scored as positive (+) if pinpoint colonies were present or as negative (−) if no colonies were seen. If large colonies appeared after 2 days of incubation, growth was given a score of 5+, and if smaller colonies appeared, growth was given a lower score proportional to the size of the colonies (e.g., 2+).